n a gag Search Results


ctt tgg cta tgg gct tcc agt idt n a adgre1 rev gca agg agg aca gag ttt atc gtg idt n a timd4  (Danaher Inc)
86
Danaher Inc ctt tgg cta tgg gct tcc agt idt n a adgre1 rev gca agg agg aca gag ttt atc gtg idt n a timd4
Ctt Tgg Cta Tgg Gct Tcc Agt Idt N A Adgre1 Rev Gca Agg Agg Aca Gag Ttt Atc Gtg Idt N A Timd4, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ctt tgg cta tgg gct tcc agt idt n a adgre1 rev gca agg agg aca gag ttt atc gtg idt n a timd4/product/Danaher Inc
Average 86 stars, based on 1 article reviews
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pcmv-gag-pol  (Cell Biolabs Inc)
90
Cell Biolabs Inc pcmv-gag-pol
Pcmv Gag Pol, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gag (slyntvatl) peptide  (GenScript corporation)
90
GenScript corporation gag (slyntvatl) peptide
Gag (Slyntvatl) Peptide, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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202220 n a pcrv gag pol prof stuart neil  (Addgene inc)
98
Addgene inc 202220 n a pcrv gag pol prof stuart neil
202220 N A Pcrv Gag Pol Prof Stuart Neil, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a gag pol addgene  (Addgene inc)
96
Addgene inc paper n a gag pol addgene
Paper N A Gag Pol Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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blyscan glycosaminoglycan assay  (Biocolor Ltd)
90
Biocolor Ltd blyscan glycosaminoglycan assay
Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated <t>glycosaminoglycan</t> [Color figure can be viewed at wileyonlinelibrary.com ]
Blyscan Glycosaminoglycan Assay, supplied by Biocolor Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blyscan glycosaminoglycan assay/product/Biocolor Ltd
Average 90 stars, based on 1 article reviews
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camk2a-tta 50-caa ccc gta aac tcg ccc aga ag-30  (Microsynth ag)
90
Microsynth ag camk2a-tta 50-caa ccc gta aac tcg ccc aga ag-30
Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated <t>glycosaminoglycan</t> [Color figure can be viewed at wileyonlinelibrary.com ]
Camk2a Tta 50 Caa Ccc Gta Aac Tcg Ccc Aga Ag 30, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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oligonucleotides siv gag f  (Eurofins)
90
Eurofins oligonucleotides siv gag f
Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated <t>glycosaminoglycan</t> [Color figure can be viewed at wileyonlinelibrary.com ]
Oligonucleotides Siv Gag F, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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expression n mlv gag pol  (Addgene inc)
91
Addgene inc expression n mlv gag pol
Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated <t>glycosaminoglycan</t> [Color figure can be viewed at wileyonlinelibrary.com ]
Expression N Mlv Gag Pol, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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ie2_flu_rev (bac mutagenesis)  (Metabion International AG)
90
Metabion International AG ie2_flu_rev (bac mutagenesis)
Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated <t>glycosaminoglycan</t> [Color figure can be viewed at wileyonlinelibrary.com ]
Ie2 Flu Rev (Bac Mutagenesis), supplied by Metabion International AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ie2_flu_rev (bac mutagenesis)/product/Metabion International AG
Average 90 stars, based on 1 article reviews
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pspax2  (Addgene inc)
90
Addgene inc pspax2
Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated <t>glycosaminoglycan</t> [Color figure can be viewed at wileyonlinelibrary.com ]
Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pspax2/product/Addgene inc
Average 90 stars, based on 1 article reviews
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gfi1-fwd gga caa gag cgt ggg cg  (Genotech Corp)
90
Genotech Corp gfi1-fwd gga caa gag cgt ggg cg
Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated <t>glycosaminoglycan</t> [Color figure can be viewed at wileyonlinelibrary.com ]
Gfi1 Fwd Gga Caa Gag Cgt Ggg Cg, supplied by Genotech Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfi1-fwd gga caa gag cgt ggg cg/product/Genotech Corp
Average 90 stars, based on 1 article reviews
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Image Search Results


Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated glycosaminoglycan [Color figure can be viewed at wileyonlinelibrary.com ]

Journal: Biotechnology and Bioengineering

Article Title: Scaffolds obtained from decellularized human extrahepatic bile ducts support organoids to establish functional biliary tissue in a dish

doi: 10.1002/bit.27613

Figure Lengend Snippet: Decellularization of human extrahepatic bile duct tissue is feasible. (a) Macroscopic images of before and after decellularization. The ostia of the PBG are visible (outtake). (b) Before and after decellularization images of H&E stained paraffin slides showing the surface of the EBD (left) and intramural PBG (right). Scale bars represent: 200 µm. (c) DAPI staining revealed that after decellularization no visible dsDNA is present in the decellularized ECM on the surface of the EBD or in the PBGS. Scale bars represent: 200 µm. (d) Scanning electron microscopy images before decellularization shows the expected denudation of the extrahepatic bile duct as only a few cells can be seen at two different magnifications from the same sample. After decellularization all cells were removed and the ultrastructure of the ECM remains intact as is shown by two different magnifications from the same sample. Scale bars represent 200 µm left side, 10 µm top right, 1 µm bottom right. (e) Collagen Type I remains present after decellularization. Scale bars represent: 200 µm. (f) Collagen type IV remains present in the PBG after decellularization. Scale bar represents: 200 µm. (g) Results from the biochemical analyses. DNA drops significantly (*** p < .001) after decellularization to 11.9 ng DNA ( SD ± 9.2 ng DNA). Total collagen content does not differ significantly ( N = 9). sGAG content decreases significantly (*** p < .001) to 1.4 µg sGAG/mg ECM ( SD ± 1.0 µg sGAG; N = 15). DAPI, 4′,6‐diamidino‐2‐phenylindole; EBD, extrahepatic bile duct; ECM, extracellular matrix; H&E, hematoxylin and eosin; PBG, peribiliary glands; sGAG, sulfated glycosaminoglycan [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Sulfated glycosaminoglycan (sGAG) was determined using a Blyscan glycosaminoglycan assay (Biocolor; N = 15).

Techniques: Staining, Electron Microscopy